DefinitionsΒΆ
- Molecular Cloning
Molecular cloning is the process of assembling together fragments of DNA to obtain a more complex molecule, often presenting genetic features of interest. It describes a process, not a technique
- GoldenGate
GoldenGate is a molecular cloning technique that uses Type IIS restriction enzymes to cut and assemble DNA sequences into recombinant DNA molecules. It describes a technique
- Modular Cloning
A Modular Cloning system uses the GoldenGate technique to assemble several genetic modules of a given level into a vector of the same level. It can also define types, which are modules or vectors with specific overhangs that are collections of sequences that are functionnally and structuraly equivalent to each other.
- MoClo
MoClo is originally the name of a modular cloning system published by the Marillonnet Lab which defines a set of vectors and modules to be used to assemble multigenic expression devices for plants. An extension was later provided by the same team proposing potentially infinite assemblies multigenic expression devices with the addition of two levels. Other modular cloning systems, inspired by them, were published under the name of MoClo (such as MoClo YTK, MoClo CIDAR, MoClo EcloFlex, etc.). In this work, the original toolkit is named MoClo IG, and MoClo is used as an abbreviation of modular cloning as defined above.