Definitions
- Molecular Cloning
- Molecular cloning is the process of assembling together fragments of DNA to
obtain a more complex molecule, often presenting genetic features of interest.
It describes a process, not a technique
- GoldenGate
- GoldenGate is a molecular cloning technique that uses Type IIS restriction
enzymes to cut and assemble DNA sequences into recombinant DNA molecules.
It describes a technique
- Modular Cloning
- A Modular Cloning system uses the GoldenGate technique to assemble several
genetic modules of a given level into a vector of the same level. It can
also define types, which are modules or vectors with specific overhangs
that are collections of sequences that are functionnally and structuraly
equivalent to each other.
- MoClo
- MoClo is originally the name of a modular cloning system published by the
Marillonnet Lab which defines a set of vectors and modules to be used to
assemble multigenic expression devices for plants. An extension was later
provided by the same team proposing potentially infinite assemblies multigenic
expression devices with the addition of two levels.
Other modular cloning systems, inspired by them, were published under the
name of MoClo (such as MoClo YTK, MoClo CIDAR, MoClo EcloFlex, etc.). In
this work, the original toolkit is named MoClo IG, and MoClo is used as an
abbreviation of modular cloning as defined above.